Gester Instruments | Professional Textile, Footwear and PPE Testing Equipments Manufacturers Since 1997
Preparation of test bacteria sheet for moisture barrier microbial penetration tester
The moisture barrier microbial penetration tester is suitable for evaluating the performance of materials in resisting the penetration of bacteria in liquids when subjected to mechanical friction. It is mainly used for medical surgical drapes, surgical gowns and clean clothes. Applicable standards: YY/T 0506.6, ENISO 22610 Main parameters: 1. Turntable speed: 60rpm±1rpm; 2. Test pressure: 3N±0.02N; 3. Outward wheel speed: 5～6rpm; 4. Timer setting range: 0～99.99min; 5. Total weight of inner and outer rings: 800g±1g. Plate preparation: Staphylococcus aureus ATCC29213 on tryptic soy agar at 36°C±Incubate at 1°C for 18-24h, inoculate 2-3 colonies into 3ml of tryptic soy broth, and inoculate at 36°C±Incubate at 1°C for 18-24h. Dilute 1:10 with peptone water to a concentration of 1×104CFU/mL~4×104CFU/mL, count the final bacterial suspension. Open the sterile bag and take out the polyurethane film still attached to the IQ, and place the wettable polyurethane film of the bacteria-carrying material sheet face up on the cleanliness plate. For ease of operation, cover the four corners of the bacteria-carrying material sheet with double-sided tape fixed on the plate. Use the petri dish cover as a template to form a corresponding area on the biofilm, apply 1.0 ml of Staphylococcus aureus suspension to this area, and then dry the bacterial sheet at 56°C for about 30min, using sterile glass during the drying period. The applicator continues to coat the bacterial suspension on the bacterial carrier film to make the bacterial liquid evenly distributed. Test principle: Put the test piece on the agar petri dish, place a piece of bacteria of the same size on the test piece, and then cover it with a piece of high-density polyethylene film with a thickness of about 10 microns, and use a tapered steel ring to seal the three-layer material ( The test material is at the bottom, the bacterial sheet is in the center, and the high-density polyethylene film is on the top), and the ring kit is placed on the agar petri dish on the turntable. The test finger acts on the material in a way that can move on the entire surface of the petri dish. , subjecting the material to the combined action of pressure and friction to simulate the possible stress and microbial penetration of the barrier material in the actual application process. The microorganisms on the bacterial sheet penetrate the test material and migrate to the surface of the agar medium. The penetration performance of the test material can be quantitatively evaluated by culturing the agar plate and counting the colonies.
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Cut resistance is one of the most critical performance indicators in personal protective equipment (PPE) testing, directly affecting worker safety in high-risk industries such as metal processing, machinery manufacturing, and emergency rescue. The TDM Cut Test Machine GT-KC28 plays a vital role in accurately evaluating the cut resistance of PPE products, including gloves, protective clothing, footwear materials, composite materials, rubber, and industrial textiles.
By adopting high-precision force control systems, intelligent data processing, and stable transmission technology, the GT-KC28 TDM Cut Tester can accurately measure the critical cutting force of materials and ensure excellent repeatability and comparability of test results. Its user-friendly touch-screen operation, comprehensive data storage, USB data export, and built-in thermal printer greatly improve laboratory efficiency and data traceability.
The TDM Cut Test Machine GT-KC28 fully complies with major international and national standards such as ISO 13997, EN 388, ASTM F2992/F2992M, ANSI/ISEA 105, and GB 24541-2022, making it a reliable solution for PPE manufacturers, third-party testing laboratories, and research institutions. Through precise and standardized cut resistance testing, the GT-KC28 helps reduce industrial cutting injuries, supports PPE certification across global markets, and ensures that protective equipment delivers reliable safety performance in real-world applications.
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