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Analysis of Test Experiment of Moisture Resistance Microbial Penetration Tester
The moisture barrier microbial penetration tester is used to determine the performance of materials in resisting the penetration of bacteria in liquids when subjected to mechanical friction (the shielding performance against penetration of bacteria carried by liquids when subjected to mechanical friction), mainly used for medical surgical drapes, surgical gowns And clean clothes, etc., (Hong Kong) Co., Ltd. is a manufacturer, and customers in need are welcome to inquire. Applicable standards: YY/T 0506.2, YY/T 0506.6-2009, EN ISO 22610 Main parameters: 1. Turntable speed: (60±1) rpm/min; 2. Test pressure: 3N±0.02N; 3. Outward wheel speed: 5～6rpm; 4. Timer setting range: 0～99.99min; 5. Total weight of inner and outer rings: 800g±1g. Test experiment: There are 5 major steps in the test experiment: The first step: preparation of bacterial slices Staphylococcus aureus ATCC29213 on tryptic soy agar at 36°C±Incubate at 1°C for 18-24h, inoculate 2-3 colonies into 3ml of tryptic soy broth, and inoculate at 36°C±Incubate at 1°C for 18-24h. Dilute 1:10 with peptone water to a concentration of 1×104CFU/mL~4×104CFU/mL, count the bacterial suspension. Open the sterile bag and take out the polyurethane film still attached to the IQ, and place the wettable polyurethane film of the bacteria-carrying material sheet face up on the cleanliness plate. For ease of operation, cover the four corners of the bacteria-carrying material sheet with double-sided tape fixed on the plate. Use the petri dish cover as a template to form a corresponding area on the biofilm, apply 1.0 ml of Staphylococcus aureus suspension to this area, and then dry the bacterial sheet at 56°C for about 30min, using sterile glass during the drying period. The applicator continues to coat the bacterial suspension on the bacterial carrier film to make the bacterial liquid evenly distributed. Use the same day after preparation. The second step: state adjustment If necessary, adjust the state of the specimen according to GB6529, or perform state and state tests under standard normal room temperature conditions. The method of state adjustment shall be recorded in the test report. The third step: test setting Adjust the weight on the control rod, so that the force value applied by the test finger to the agar is 3N±0.02N. Place an agar petri dish on the turntable. Step 4: Material Application Use the following counts: Use a circular weight composed of inner and outer rings, with a total weight of 800g±1g applies a standard taut force to the material. Place the cylinder in the center of the inner ring, then cover the test piece on the cylinder and the inner ring, and place the stained side of the bacterial sheet after removing the sticker paper down on the test piece. Cover a layer of HDPE film on the polyurethane film, push down the outer ring tightly, so that the three-layer material is firmly added between the two rings. Step 5: Carry out the test. Gently place the above ring assembly on an agar petri dish with the lid removed. The steel ring is freely suspended on the outside of the rotating disk. This allows the test piece to come into contact with the agar surface. The test finger is to apply 3N pressure according to the above regulations, and the instrument runs for 15min. Remove the ring kit immediately after 15 minutes and set aside. Remove a petri dish from the carousel and place the lid on. Immediately place the second Petri dish and ring kit on the rotating plate. Perform the above steps with the same ring assembly for the next four Petri dishes. After the five petri dishes have completed the test, remove the bacterial slices and discard them, invert the specimen, and cover it with HDPE film upside down. Operate on the sixth petri dish for 15 minutes to complete a parallel test group. For the remaining 4 specimens, run six petri dishes for 15 min each according to the above method, and use a freshly prepared bacterial slice for each specimen. If there is liquid accumulated on the surface of the agar, put it on a clean bench to dry, cover each agar petri dish and place it at 36°C±Incubate at 1°C for 48h. Count the number of Staphylococcus aureus colonies in each petri dish, and the number of colonies within a radius of 15 minutes from the center of the petri dish is not counted.
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